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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
30/06/2020 |
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Data da última atualização: |
30/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROBERTS, A.; BOECKMAN, C. J.; MÜHL, M.; ROMEIS, J.; TEEM, J. L.; VALICENTE, F. H.; BROWN, J. K.; EDWARDS, M. G.; LEVINE, S. L.; MELNICK, R. L.; ROFRIGUES, T. B.; VÉLEZ, A. M.; ZHOU, X.; HELLMICH, R. L. |
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Afiliação: |
Andrew Roberts, Agriculture and Food Systems Institute, Washington, DC; Chad J. Boeckman, Corteva Agriscience; Marina Mühl, Ministerio de Agricultura, Ganadería y Pesca, Dirección de Biotecnología, Buenos Aires; Jörg Romeis, Research Division Agroecology and Environment, Agroscope, Zurich; John L. Teem, Agriculture and Food Systems Institute, Washington, DC; FERNANDO HERCOS VALICENTE, CNPMS; Judith K. Brown, School of Plant Sciences, The University of Arizona, Tucson; Martin G. Edwards, School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne; Steven L. Levine, Bayer Crop Science, Chesterfield, MO; Rachel L. Melnick, Agriculture and Food Systems Institute, Washington, DC; Thais B. Rodrigues, Greenlight Biosciences, Inc., Medford, MA; Ana M. Vélez, Department of Entomology, University of Nebraska-Lincoln; Xuguo Zhou, Department of Entomology, University of Kentucky, Lexington, KY; Richard L. Hellmich, USDA, Corn Insects and Crop Genetics Research Unit, Ames. |
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Título: |
Sublethal endpoints in non-target organism testing for insect-active GE crops. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Bioengineering and Biotechnology, v. 8, article 556, 2020. |
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DOI: |
https://doi.org/10.3389/fbioe.2020.00556 |
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Idioma: |
Inglês |
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Conteúdo: |
Historically, genetically engineered (GE) plants that have incorporated genes conferring insect protection have primarily used Cry proteins derived from Bacillus thuringiensis (Bt) to achieve their insecticidal phenotype. As a result, regulators have developed a level of familiarity and confidence in reviewing plants incorporating these insecticidal proteins. However, new technologies have been developed that produce GE plants that incorporate pest protection by triggering an RNA interference (RNAi) response or proteins other than Bt Cry proteins. These technologies have new modes of action. Although the overall assessment paradigm for GE plants is robust, there are ongoing discussions about the appropriate tests and measurement endpoints needed to inform non-target arthropod assessment for technologies that have a different mode of action than the Bt Cry proteins. As a result, increasing attention is being paid to the use of sublethal endpoints and their value for environmental risk assessment (ERA). This review focuses on the current status and history of sublethal endpoint use in insect-active GE crops, and evaluates the future use of sublethal endpoints for new and emerging technologies. It builds upon presentations made at the Workshop on Sublethal Endpoints for Non-target Organism Testing for Non-Bt GE Crops (Washington DC, USA, 4â??5 March 2019), and the discussions of government, academic and industry scientists convened for the purpose of reviewing the progress and status of sublethal endpoint testing in non-target organisms. MenosHistorically, genetically engineered (GE) plants that have incorporated genes conferring insect protection have primarily used Cry proteins derived from Bacillus thuringiensis (Bt) to achieve their insecticidal phenotype. As a result, regulators have developed a level of familiarity and confidence in reviewing plants incorporating these insecticidal proteins. However, new technologies have been developed that produce GE plants that incorporate pest protection by triggering an RNA interference (RNAi) response or proteins other than Bt Cry proteins. These technologies have new modes of action. Although the overall assessment paradigm for GE plants is robust, there are ongoing discussions about the appropriate tests and measurement endpoints needed to inform non-target arthropod assessment for technologies that have a different mode of action than the Bt Cry proteins. As a result, increasing attention is being paid to the use of sublethal endpoints and their value for environmental risk assessment (ERA). This review focuses on the current status and history of sublethal endpoint use in insect-active GE crops, and evaluates the future use of sublethal endpoints for new and emerging technologies. It builds upon presentations made at the Workshop on Sublethal Endpoints for Non-target Organism Testing for Non-Bt GE Crops (Washington DC, USA, 4â??5 March 2019), and the discussions of government, academic and industry scientists convened for the purpose of reviewing the progress and ... Mostrar Tudo |
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Palavras-Chave: |
Organismos não-alvo; Parâmetros subletais; Planta geneticamente modificada. |
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Thesagro: |
Controle Biológico; Inseto; Melhoramento Genético Vegetal; Planta Transgênica. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/214276/1/Sublethal-endpoints.pdf
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Marc: |
LEADER 02669naa a2200373 a 4500
001 2123519
005 2020-06-30
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fbioe.2020.00556$2DOI
100 1 $aROBERTS, A.
245 $aSublethal endpoints in non-target organism testing for insect-active GE crops.$h[electronic resource]
260 $c2020
520 $aHistorically, genetically engineered (GE) plants that have incorporated genes conferring insect protection have primarily used Cry proteins derived from Bacillus thuringiensis (Bt) to achieve their insecticidal phenotype. As a result, regulators have developed a level of familiarity and confidence in reviewing plants incorporating these insecticidal proteins. However, new technologies have been developed that produce GE plants that incorporate pest protection by triggering an RNA interference (RNAi) response or proteins other than Bt Cry proteins. These technologies have new modes of action. Although the overall assessment paradigm for GE plants is robust, there are ongoing discussions about the appropriate tests and measurement endpoints needed to inform non-target arthropod assessment for technologies that have a different mode of action than the Bt Cry proteins. As a result, increasing attention is being paid to the use of sublethal endpoints and their value for environmental risk assessment (ERA). This review focuses on the current status and history of sublethal endpoint use in insect-active GE crops, and evaluates the future use of sublethal endpoints for new and emerging technologies. It builds upon presentations made at the Workshop on Sublethal Endpoints for Non-target Organism Testing for Non-Bt GE Crops (Washington DC, USA, 4â??5 March 2019), and the discussions of government, academic and industry scientists convened for the purpose of reviewing the progress and status of sublethal endpoint testing in non-target organisms.
650 $aControle Biológico
650 $aInseto
650 $aMelhoramento Genético Vegetal
650 $aPlanta Transgênica
653 $aOrganismos não-alvo
653 $aParâmetros subletais
653 $aPlanta geneticamente modificada
700 1 $aBOECKMAN, C. J.
700 1 $aMÜHL, M.
700 1 $aROMEIS, J.
700 1 $aTEEM, J. L.
700 1 $aVALICENTE, F. H.
700 1 $aBROWN, J. K.
700 1 $aEDWARDS, M. G.
700 1 $aLEVINE, S. L.
700 1 $aMELNICK, R. L.
700 1 $aROFRIGUES, T. B.
700 1 $aVÉLEZ, A. M.
700 1 $aZHOU, X.
700 1 $aHELLMICH, R. L.
773 $tFrontiers in Bioengineering and Biotechnology$gv. 8, article 556, 2020.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
21/12/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
FREITAS, D. S.; TERRY, S. A.; RIBEIRO, R. S.; PEREIRA, L. G. R.; TOMICH, T. R.; MACHADO, F. S.; CAMPOS, M. M.; CORRÊA, P. S.; ABDALLA, A. L.; MAURÍCIO, R. M.; CHAVES, A. V. |
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Afiliação: |
DANIELLE S. FREITAS, UFSJ; STEPHANIE A. TERRY, University of Sydney, Sydney, Australia; RAFAEL S. RIBEIRO, UFSJ; LUIZ GUSTAVO RIBEIRO PEREIRA, CNPGL; THIERRY RIBEIRO TOMICH, CNPGL; FERNANDA SAMARINI MACHADO, CNPGL; MARIANA MAGALHAES CAMPOS, CNPGL; PATRICIA S. CORRÊA, USP; ADIBE L. ABDALLA, USP; ROGÉRIO M. MAURÍCIO, UFSJ; ALEXANDRE V. CHAVES, University of Sydney, Sydney, Australia. |
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Título: |
Unconventional vegetable oils for a reduction of methanogenesis and modulation of ruminal fermentation. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Frontiers in Veterinary Science, v. 5, article 201, 2018. |
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Páginas: |
9 p. |
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DOI: |
10.3389/fvets.2018.00201 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to evaluate the use of vegetable oils from plants grown in Brazil, first using the in vitro batch culture, and then evaluating the oil with methane (CH4) reducing potential in an in vivo experiment. The in vitro experiment was conducted as a completely randomized design using the seven contrasting oils. Treatments consisted of a control and 3 increasing concentrations (0, 1, 2, and 5% v/v) of oil added to a tifton 85 hay samples. All vegetable oils linearly decreased (P < 0.01) gas production after 24 h of incubation, with the greatest reduction when 5% of oil was included into the diet. Açaí and buriti had no effect of CH4 (% or mL/g DM incubated) however carrot, macaúba, basil, passionflower, and pequi oil all linearly decreased (P < 0.01) CH4 production with increasing inclusion rate of oil. Pequi oil resulted in the largest decrease in CH4 production (mL/g DM incubated) after 24 h of in vitro incubation. The objective of the in vivo experiment was to evaluate the effects of pequi oil on nutrient digestibility, CH4 production, and rumen fermentation parameters in wethers fed a hay-based diet. The experiment was conducted as a 2 × 2 Latin Square design using 4 Dorper wethers (63.4 ± 1.46 kg body weight). There were 2 experimental periods of 21 d each, with d 1?14 used for diet adaptation and d 15?21 for measurements and collections. The treatments consisted of a control diet and pequi oil fed at 70 g per animal per day. The addition of pequi oil to the diet had no effect on feed intake or the digestibility of nutrients, however there was a numerical decrease in the population of cellulolytic bacteria. There was a tendency (P = 0.06) for pequi oil addition to decrease CH4 production (g/d) by 17.5%. From this study, we can conclude that pequi oil may be used as a suitable oil for reducing CH4 production from ruminants, with no negative effects on intake or digestibility. MenosThe objective of this study was to evaluate the use of vegetable oils from plants grown in Brazil, first using the in vitro batch culture, and then evaluating the oil with methane (CH4) reducing potential in an in vivo experiment. The in vitro experiment was conducted as a completely randomized design using the seven contrasting oils. Treatments consisted of a control and 3 increasing concentrations (0, 1, 2, and 5% v/v) of oil added to a tifton 85 hay samples. All vegetable oils linearly decreased (P < 0.01) gas production after 24 h of incubation, with the greatest reduction when 5% of oil was included into the diet. Açaí and buriti had no effect of CH4 (% or mL/g DM incubated) however carrot, macaúba, basil, passionflower, and pequi oil all linearly decreased (P < 0.01) CH4 production with increasing inclusion rate of oil. Pequi oil resulted in the largest decrease in CH4 production (mL/g DM incubated) after 24 h of in vitro incubation. The objective of the in vivo experiment was to evaluate the effects of pequi oil on nutrient digestibility, CH4 production, and rumen fermentation parameters in wethers fed a hay-based diet. The experiment was conducted as a 2 × 2 Latin Square design using 4 Dorper wethers (63.4 ± 1.46 kg body weight). There were 2 experimental periods of 21 d each, with d 1?14 used for diet adaptation and d 15?21 for measurements and collections. The treatments consisted of a control diet and pequi oil fed at 70 g per animal per day. The addition of pequi... Mostrar Tudo |
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Palavras-Chave: |
Cerrado biome; Exotic oils; In vitro. |
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Thesaurus NAL: |
Methane; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189260/1/Artigo-Frontiers-in-Microb-LuizG-Unconventional.pdf
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Marc: |
LEADER 02858naa a2200325 a 4500
001 2102430
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.3389/fvets.2018.00201$2DOI
100 1 $aFREITAS, D. S.
245 $aUnconventional vegetable oils for a reduction of methanogenesis and modulation of ruminal fermentation.$h[electronic resource]
260 $c2018
300 $a9 p.
520 $aThe objective of this study was to evaluate the use of vegetable oils from plants grown in Brazil, first using the in vitro batch culture, and then evaluating the oil with methane (CH4) reducing potential in an in vivo experiment. The in vitro experiment was conducted as a completely randomized design using the seven contrasting oils. Treatments consisted of a control and 3 increasing concentrations (0, 1, 2, and 5% v/v) of oil added to a tifton 85 hay samples. All vegetable oils linearly decreased (P < 0.01) gas production after 24 h of incubation, with the greatest reduction when 5% of oil was included into the diet. Açaí and buriti had no effect of CH4 (% or mL/g DM incubated) however carrot, macaúba, basil, passionflower, and pequi oil all linearly decreased (P < 0.01) CH4 production with increasing inclusion rate of oil. Pequi oil resulted in the largest decrease in CH4 production (mL/g DM incubated) after 24 h of in vitro incubation. The objective of the in vivo experiment was to evaluate the effects of pequi oil on nutrient digestibility, CH4 production, and rumen fermentation parameters in wethers fed a hay-based diet. The experiment was conducted as a 2 × 2 Latin Square design using 4 Dorper wethers (63.4 ± 1.46 kg body weight). There were 2 experimental periods of 21 d each, with d 1?14 used for diet adaptation and d 15?21 for measurements and collections. The treatments consisted of a control diet and pequi oil fed at 70 g per animal per day. The addition of pequi oil to the diet had no effect on feed intake or the digestibility of nutrients, however there was a numerical decrease in the population of cellulolytic bacteria. There was a tendency (P = 0.06) for pequi oil addition to decrease CH4 production (g/d) by 17.5%. From this study, we can conclude that pequi oil may be used as a suitable oil for reducing CH4 production from ruminants, with no negative effects on intake or digestibility.
650 $aMethane
650 $aSheep
653 $aCerrado biome
653 $aExotic oils
653 $aIn vitro
700 1 $aTERRY, S. A.
700 1 $aRIBEIRO, R. S.
700 1 $aPEREIRA, L. G. R.
700 1 $aTOMICH, T. R.
700 1 $aMACHADO, F. S.
700 1 $aCAMPOS, M. M.
700 1 $aCORRÊA, P. S.
700 1 $aABDALLA, A. L.
700 1 $aMAURÍCIO, R. M.
700 1 $aCHAVES, A. V.
773 $tFrontiers in Veterinary Science$gv. 5, article 201, 2018.
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